Phytophthora antagonism of endophytic bacteria isolated from roots of black pepper (Piper nigrum L.)

doi:10.3390/agronomy10020286

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Title:	Phytophthora antagonism of endophytic bacteria isolated from roots of black pepper (Piper nigrum L.)
Authors:	Van Anh Ngo, San-Lang Wang, Van Bon Nguyen, Chien Thang Doan, Thi Ngoc Tran, Dinh Minh Tran, Trung Dzung Tran, Anh Dzung Nguyen
Keywords:	endophytic bacteria;black pepper;Bacillus velezensis / siamensis/ methylotrophycus;antifungal activity;Phytophthora
Date:	2020-02-17
Issue Date:	2020-02-19 12:10:12 (UTC+8)
Publisher:	MDPI
Abstract:	Abstract: In this study, 90 root samples were collected from 30 black pepper farms in three provinces in the Central Highlands of Vietnam. A total of 352 endophytic bacteria were isolated and their morphology described. An in vitro assay on the ntifungal activity of these isolates was then conducted and 47 isolates were found to have antagonistic activity on Phytophthora fungi. The antifungal activity of the 47 isolates was evaluated in vivo by shoot assay. Among these 47 isolates, 6 were selected for further investigation. The six isolates were classified and identified by sequencing the 16S RNA gene and phylogeny. The results showed that all six endophytic bacteria belong to the following species of Bacillus genus: B. siamensis, B. amyloliquefaciens, B. velezenis, and B. methylotrophiycus. Enzymatic activity related to the antifungal activity of the six potent isolates was determined; it showed that they possessed high chitinase and protease activities. These isolates were applied for black pepper seedlings in greenhouse. The results showed three promising isolates: B. siamensis EB.CP6, B. velezensis EB.KN12, and B. methylotrophycus EB.KN13. Black pepper seedlings treated with the promising bacteria had the lowest rate of root disease (8.45–11.21%) and lower fatal rate (11.11–15.55%) compared to the control group (24.81% and 24.44%). In addition, the three promising isolates strongly affected the growth of the black pepper seedlings in greenhouse. The plant height, length of roots, and fresh biomass of the seedlings in the treated plots were higher than those in the control plots. Thus, the endophytic bacterial isolates have the potential to act as biocontrol agent for the sustainable production of black pepper.
Relation:	Agronomy 10(2), 286
DOI:	10.3390/agronomy10020286
Appears in Collections:	[Graduate Institute & Department of Chemistry] Journal Article

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