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    Please use this identifier to cite or link to this item: http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/118053


    Title: Novel Efficient Bioprocessing of Marine Chitins into Active Anticancer Prodigiosin
    Authors: Nguyen, Van Bon;Chen, Shan-Ping;Nguyen, Thi Hanh;Nguyen, Minh Trung;Tran, Thi Thanh Thao;Doan, Chien Thang;Tran, Thi Ngoc;Nguyen, Anh Dzung;Kuo, Yao-Haur;Wang, San-Lang
    Keywords: Serratia marcescens;anti-tumors;bioprocessing;prodigiosin;α-Chitin
    Date: 2019-12-23
    Issue Date: 2020-01-21 12:10:54 (UTC+8)
    Publisher: MDPI
    Abstract: Marine chitins (MC) have been utilized for the production of vast array of bioactive products,
    including chitooligomers, chitinase, chitosanase, antioxidants, anti-NO, and antidiabetic compounds.
    The aim of this study is the bioprocessing of MC into a potent anticancer compound, prodigiosin (PG),
    via microbial fermentation. This bioactive compound was produced by Serratia marcescens TKU011
    with the highest yield of 4.62 mg/mL at the optimal conditions of liquid medium with initial pH of
    5.65–6.15 containing 1% -chitin, 0.6% casein, 0.05% K2HPO4, and 0.1% CaSO4. Fermentation was
    kept at 25 C for 2 d. Notably, -chitin was newly investigated as the major potential material for PG
    production via fermentation; the salt CaSO4 was also found to play the key role in the enhancement of
    PG yield of Serratia marcescens fermentation for the first time. PG was qualified and identified based
    on specific UV, MALDI-TOF MS analysis. In the biological activity tests, purified PG demonstrated
    potent anticancer activities against A549, Hep G2, MCF-7, and WiDr with the IC50 values of 0.06,
    0.04, 0.04, and 0.2 g/mL, respectively. Mytomycin C, a commercial anti-cancer compound was also
    tested for comparison purpose, showing weaker activity with the IC50 values of 0.11, 0.1, 0.14, and
    0.15 g/mL, respectively. As such, purified PG displayed higher 2.75-fold, 1.67-fold, and 3.25-fold
    ecacy than Mytomycin C against MCF-7, A549, and Hep G2, respectively. The results suggest
    that marine chitins are valuable sources for production of prodigiosin, a potential candidate for
    cancer drugs.
    Relation: Marine Drugs 2020, 18, 15
    DOI: 10.3390/md18010015
    Appears in Collections:[Graduate Institute & Department of Chemistry] Journal Article

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