Conversion of shrimp head waste for production of a thermotolerant, detergent-stable, alkaline protease by Paenibacillus sp.

淡江大學機構典藏 > 理學院 > 化學學系暨研究所 > 期刊論文 > Item 987654321/117974

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Title:	Conversion of shrimp head waste for production of a thermotolerant, detergent-stable, alkaline protease by Paenibacillus sp.
Authors:	Doan CT, Tran TN, Wen IH, Nguyen VB, Nguyen AD, Wang SL
Keywords:	marine chitinous by-products;Paenibacillus;protease;shrimp heads
Date:	2019-09-24
Issue Date:	2020-01-07 12:11:04 (UTC+8)
Publisher:	MDPI
Abstract:	Fishery processing by-products have been of great interest to researchers due to their beneficial applications in many fields. In this study, five types of marine by-products, including demineralized crab shell, demineralized shrimp shell, shrimp head, shrimp shell, and squid pen, provided sources of carbon and nitrogen nutrition by producing a protease from Paenibacillus sp. TKU047. Strain TKU047 demonstrated the highest protease productivity (2.98 U/mL) when cultured for two days on a medium containing 0.5% of shrimp head powder (SHP). The mass of TKU047 protease was determined to be 32 kDa (approximately). TKU047 protease displayed optimal activity at 70–80 °C and pH 9, with a pH range of stability from 6 to 11. TKU047 protease also showed stability in solutions containing surfactants and detergents. Based on its excellent properties, Paenibacillus sp. TKU047 protease may be a feasible candidate for inclusion in laundry detergents.
Relation:	Catalysts 2019 9(10), p.798
DOI:	10.3390/catal9100798
Appears in Collections:	[化學學系暨研究所] 期刊論文

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