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    請使用永久網址來引用或連結此文件: https://tkuir.lib.tku.edu.tw/dspace/handle/987654321/117140

    題名: Reclamation of rhizobacteria newly isolated from black pepper plant roots as potential biocontrol agents of root-knot nematodes
    作者: Van Bon Nguyen;San-Lang Wang;Thi Hanh Nguyen;Thi Huyen Nguyen;Thi Huyen Trang Trinh;Thi Thiep Nong;To Uyen Nguyen;Van Nam Nguyen;Anh Dzung Nguyen
    關鍵詞: Root-knot nematodes;Meiloidogyne spp.;Pepper;Biocontrol agents;Bacillus subtilis
    日期: 2019-11
    上傳時間: 2019-09-26 12:10:57 (UTC+8)
    出版者: Springers
    摘要: Rhizobacteria have been of great interest due to their promising use in biocontrol and promotion of plant growth. The aim of this study was to select active anti-nematode rhizobacteria, enhance their nematicidal activity by optimizing fermentation conditions, and then determine the active components of the fermented product. Among more than 100 isolates, 20 strains showed potent inhibition (81.67–99%) against root-knot nematodes from the genus Meiloidogyne. Almost all of these belong to the genus Bacillus (14 of 20 strains). The six most active strains (with max inhibition of 96.13–98.33%) were RB.DS.05, RB.DL.28, RB.DS.29, RB.DL.31, H11, and CC01; these strains were further investigated for their effect on nematode egg hatching. Of these, Bacillus subtilis RB.DL.28 demonstrated the greatest inhibition (82%). Chitinase and protease were closely associated with the inhibition of egg hatching, while it was newly discovered that natural compounds with thermal stability play a key role in killing J2 nematodes. Based on LC–MS analysis, two major constituents, sulfacetamide and metronidazole-OH, were identified from the filtrates of B. subtilis RB.DL.28. The results from this study suggest that the most active strain, B. subtilis RB.DL.28, and other newly identified rhizobacteria may be good candidates for biocontrol of Meiloidogyne spp. These results expand the catalogue of novel active anti-nematode rhizobacteria communities recently recorded in Vietnam.
    關聯: Research on Chemical Intermediates 45(11),p.5293-5307
    DOI: 10.1007/s11164-019-03970-6
    顯示於類別:[化學學系暨研究所] 期刊論文


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