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    Please use this identifier to cite or link to this item: http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/117039

    Title: Embryonic exposure to 4-methylimidazole leads to zebrafish myofibril misalignment
    Authors: Tsai, Jen‐Ning;Sun, Chiao‐Yin;Ding, Yu‐Ju;Wang, Yun‐Hsin;Wen, Kang‐Chieh Lo Chi‐Chung;Lin, Jia‐Wei;Chang, Chiung‐Fang;Hsu, Li‐Sung;Chen, Hui‐Min;Fong, Tsorng‐Harn;Chen, Yau‐Hung
    Keywords: 4‐methylimidazole;myogenesis;zebrafish
    Date: 2018-12
    Issue Date: 2019-09-17 12:12:51 (UTC+8)
    Abstract: 4‐methylimidazole (4‐MI) is an imidazole‐derived organic chemical compound that can be used as a raw material in the manufacture of diverse chemicals and has been identified as an ingredient of caramel color in soybean sauce, beers, and other soft drinks. The aim of the present study was to investigate the teratogenic effects of 4‐MI during zebrafish embryogenesis. Zebrafish embryos were treated with different dosages of 4‐MI (0‐120 mM) for different exposure durations (12‐60 hours). The percentages of embryos with malformed phenotypes increased as the exposure dosages and duration time of 4‐MI increased. We also used immunofluorescence and transmission microscopy to evaluate the subtle changes in the myofibril alignment and ultrastructure of muscle organization. Our data showed that 4‐MI treatment disturbs muscle fiber alignment. Electron microscopy data indicated that Z‐lines were undetectable in the 4‐MI‐treated embryos. Although the thick and thin filaments were visible, they were all disorganized. In addition, zebrafish embryos treated by 4‐MI exhibited aberrant expression of 2 muscle‐specific genes, myod and myogenin. Taken together, we concluded that early exposure to 4‐MI affects zebrafish myogenesis, especially in myofibril alignment.
    Relation: Environmental Toxicology 33(12), p.1321-1328
    DOI: 10.1002/tox.22640
    Appears in Collections:[Graduate Institute & Department of Chemistry] Journal Article

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