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    Please use this identifier to cite or link to this item: http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/115557

    Title: Reclamation of Marine Chitinous Materials for Chitosanase Production via Microbial Conversion by Paenibacillus macerans
    Authors: Chien Thang Doan;Thi Ngoc Tran;Van Bon Nguyen;Anh Dzung Nguyen;San-Lang Wang
    Keywords: chitin;chitosan;protease;chitinase;chitosan oligomers
    Date: 2018-11-02
    Issue Date: 2018-11-08 12:12:01 (UTC+8)
    Publisher: MDPI
    Abstract: : Chitinous materials from marine byproducts elicit great interest among biotechnologists for
    their potential biomedical or agricultural applications. In this study, four kinds of marine chitinous
    materials (squid pens, shrimp heads, demineralized shrimp shells, and demineralized crab shells)
    were used to screen the best source for producing chitosanase by Paenibacillus macerans TKU029.
    Among them, the chitosanase activity was found to be highest in the culture using the medium
    containing squid pens as the sole carbon/nitrogen (C/N) source. A chitosanase which showed
    molecular weights at 63 kDa was isolated from P. macerans cultured on a squid pens medium.
    The purified TKU029 chitosanase exhibited optimum activity at 60 ◦C and pH 7, and was stable at
    temperatures under 50 ◦C and pH 3-8. An analysis by MALDI-TOF MS revealed that the chitosan
    oligosaccharides (COS) obtained from the hydrolysis of water-soluble chitosan by TKU029 crude
    enzyme showed various degrees of polymerization (DP), varying from 3–6. The obtained COS
    enhanced the growth of four lactic acid bacteria strains but exhibited no effect on the growth of E. coli.
    By specialized growth enhancing effects, the COS produced from hydrolyzing water soluble chitosan
    with TKU029 chitinolytic enzymes could have potential for use in medicine or nutraceuticals.
    Relation: Marine Drugs 16(11), 429
    Appears in Collections:[化學學系暨研究所] 期刊論文

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