淡江大學機構典藏:Item 987654321/114253
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    题名: Staphylococcus sp. TKU043 所生產α-葡萄糖苷酶抑制劑之研究
    其它题名: Studies on the production of α-glucosidase inhibitors by Staphylococcus sp. TKU043
    作者: 蘇育正;Su, Yu-Cheng
    贡献者: 淡江大學化學學系碩士班
    王三郎;Wang, San-Lang
    关键词: alpha-glucosidase inhibitors;diabetes mellitus;shrimp head powder;Staphylococcus;α-葡萄糖苷酶抑制劑;葡萄球菌;蝦頭殼粉;糖尿病
    日期: 2017
    上传时间: 2018-08-03 14:46:37 (UTC+8)
    摘要: α-葡萄糖苷酶抑制劑由於其在治療糖尿病中的重要用途而受到重視。雖然一些合成的 α-葡萄糖苷酶抑制劑已經可以使用很長時間，但是它們經常會引起各種的副作用。因此，本研究旨在找到一種安全，天然的 α-葡萄糖苷酶抑制劑來源。本實驗利用篩選自淡江大學校園之菌株Staphylococcus sp. TKU 043，發酵蝦頭殼粉生產α-葡萄糖苷酶抑制劑。於含1%蝦頭殼粉培養基培養 3 天，可得較佳α-葡萄糖苷酶抑制活性（1457U / mL）。所得發酵上清液經硫酸銨鹽沉澱、DEAE-Sepharose陰離子交換管柱層析、Sepharcryl-S200膠體管柱層析，進行α-葡萄糖苷酶抑制劑之部份純化。此抑制劑於50℃處理30 分鐘後活性僅存20％，在pH 3-7反應皆具100 %之抑制活性。
    　　依據研究結果顯示，TKU 043所生產之α-葡萄糖苷酶抑制劑為大分子化合物，推測此抑制劑可能為蛋白質類或是胜肽類化合物；利用酵素抑制動力學鑑定出此抑制劑為混合型(非競爭)抑制劑。
    Alpha-glucosidase inhibitors have received much attention due to their important use in treating diabetes mellitus. Although some synthetic α-glucosidase inhibitors have been available for a long time, they often cause various unexpected side effects. Thus, the present study was aimed at finding a safe, natural source of α-glucosidase inhibitors. Staphylococcus sp. TKU043, a bacterium strain isolated from the soils of Tamkang University Campus, produced alpha-glucosidase inhibitors (1457 U/mL) in a medium with 1% (w/v) shrimp head powder as the sole carbon/nitrogen source. The culture supernatant obtained by culturing under optimized culture condition was purified by ammonium sulfate precipitation, DEAE-Sepharose column chromatography, and Sephacryl S-200 gel chromatography. The partially purified inhibitors remained 20 % original activity after treatment at 50℃ for 30 min. Activity of the alpha-glucosidase inhibitors remained 100 % after treatment at pH 3-7.
    　　According to the results of this study, the α-glucosidase inhibitor produced by TKU043 is a proteinous material. The inhibitor is identified as a mixed type (non-competitive) inhibitor according to the results of enzyme inhibition kinetics.
    显示于类别:[化學學系暨研究所] 學位論文

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