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    Please use this identifier to cite or link to this item: https://tkuir.lib.tku.edu.tw/dspace/handle/987654321/113399


    Title: Production and bioactivity-guided isolation of antioxidants with α-glucosidase inhibitory and anti-NO properties from marine chitinous materials
    Authors: Van Bon Nguyen;Thi Hanh Nguyen;Chien Thang Doan;Thi Ngoc Tran;Anh Dzung Nguyen;Yao-Haur Kuo;San-Lang Wang
    Keywords: antioxidants;anti-inflammation;chitin;exopolysaccharides;homogentisic acid
    Date: 2018-05-09
    Issue Date: 2018-05-11 12:10:15 (UTC+8)
    Publisher: MDPI
    Abstract: Natural and bioactive products have been of great interest due to their benefit as
    health foods and drugs to prevent various diseases. The aim of this study is to efficiently reuse
    marine chitinous materials (CMs), abundant and low-cost fishery by-products, for the bio-synthesis,
    isolation, and identification of antioxidant compounds possessing some other beneficial bioactivities.
    Paenibacillus sp. was used to convert CMs to antioxidants. Among various tested CMs, squid pen
    powder (SPP) gave the best results when used as the sole carbon/nitrogen source. Fermented
    SPP (FSPP) had comparable antioxidant activity (IC50 = 124 µg/mL) to that of α-tocopherol
    (IC50 = 30 µg/mL). The antioxidant productivity increased 1.83-fold after culture optimization.
    The use of multiple techniques, including Diaion, silica, and preparative HPLC columns coupled with
    a bioassay resulted in the isolation of two major antioxidants characterized as exopolysaccharides
    and homogentisic acid. These isolated compounds showed great maximum activity and low
    IC50 values (96%, 30 µg/mL and 99%, 5.4 µg/mL, respectively) which were comparable to that
    of α-tocopherol (95%, 24 µg/mL). The crude sample, fractions, and isolated compounds also
    demonstrated α-glucosidase inhibition and anti–inflammatory activity. Notably, homogentisic
    acid was found as a non-sugar-based moiety α-glucosidase inhibitor which show much higher
    inhibition (IC50 = 215 µg/mL) than that of acarbose (IC50 = 1324 µg/mL) and also possessed
    acceptable anti–inflammatory activity (IC50 = 9.8 µg/mL). The results highlighted the value of
    using seafood processing by-products, like squid pens, for the production of several compounds
    possessing multi-benefit bioactivities and no cytotoxicity.
    Relation: Molecules 23(5), 1124(14 pages)
    DOI: 10.3390/molecules23051124
    Appears in Collections:[Graduate Institute & Department of Chemistry] Journal Article

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