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    題名: Conversion of squid pens to chitosanases and proteases via Paenibacillus sp TKU042
    作者: Doan CT;Tran TN;Nguyen VB;Nguyen AD;Wang SL
    關鍵詞: Paenibacillus;chitin;chitosan;chitosanase;protease;squid pens;α-glucosidase inhibitors
    日期: 2018-03
    上傳時間: 2018-03-15 12:10:54 (UTC+8)
    出版者: MDPI
    摘要: Chitosanases and proteases have received much attention due to their wide range of applications. Four kinds of chitinous materials, squid pens, shrimp heads, demineralized shrimp shells and demineralized crab shells, were used as the sole carbon and nitrogen (C/N) source to produce chitosanases, proteases and α-glucosidase inhibitors (αGI) by four different strains of Paenibacillus. Chitosanase productivity was highest in the culture supernatants using squid pens as the sole C/N source. The maximum chitosanase activity of fermented squid pens (0.759 U/mL) was compared to that of fermented shrimp heads (0.397 U/mL), demineralized shrimp shells (0.201 U/mL) and demineralized crab shells (0.216 U/mL). A squid pen concentration of 0.5% was suitable for chitosanase, protease and αGI production via Paenibacillus sp. TKU042. Multi-purification, including ethanol precipitation and column chromatography of Macro-Prep High S as well as Macro-Prep DEAE (diethylaminoethyl), led to the isolation of Paenibacillus sp. TKU042 chitosanase and protease with molecular weights of 70 and 35 kDa, respectively. For comparison, 16 chitinolytic bacteria, including strains of Paenibacillus, were investigated for the production of chitinase, exochitinase, chitosanase, protease and αGI using two kinds of chitinous sources.
    關聯: Marine Drugs 16(83), pp.1-12
    DOI: 10.3390/md16030083
    顯示於類別:[化學學系暨研究所] 期刊論文


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