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    Please use this identifier to cite or link to this item: http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/111839

    Title: Utilization of Fishery Processing By-Product Squid Pens for α-Glucosidase Inhibitors Production by Paenibacillus sp.
    Authors: Nguyen Van Bon;Nguyen Anh Dzung;Wang San-Lang
    Keywords: squid pens;α-glucosidase inhibitor;Paenibacillus species;diabetes;obesity;microbial conversion;fishery processing;homogentisic acid
    Date: 2017/08/30
    Issue Date: 2017-10-25 02:11:20 (UTC+8)
    Publisher: MDPI
    Abstract: The supernatants (the solution part received after centrifugation) of squid pens fermented
    by four species of Paenibacillus showed potent inhibitory activity against α-glucosidases derived
    from yeast (79–98%) and rats (76–83%). The inhibition of acarbose—a commercial antidiabetic
    drug, used against yeast and rat α-glucosidases—was tested for comparison; it showed inhibitory
    activity of 64% and 88%, respectively. Other chitinolytic or proteolytic enzyme-producing bacterial
    strains were also used to ferment squid pens, but no inhibition activity was detected from the
    supernatants. Paenibacillus sp. TKU042, the most active α-glucosidase inhibitor (aGI)-producing
    strain, was selected to determine the optimal cultivation parameters. This bacterium achieved the
    highest aGI productivity (527 µg/mL) when 1% squid pens were used as the sole carbon/nitrogen
    source with a medium volume of 130 mL (initial pH 6.85) in a 250 mL flask (48% of air head space), at
    30 ◦C for 3–4 d. The aGI productivity increased 3.1-fold after optimization of the culture conditions.
    Some valuable characteristics of Paenibacillus aGIs were also studied, including pH and thermal
    stability and specific inhibitory activity. These microbial aGIs showed efficient inhibition against
    α-glucosidases from rat, yeast, and bacteria, but weak inhibition against rice α-glucosidase with IC50
    values of 362, 252, 189, and 773 µg/mL, respectively. In particular, these aGIs showed highly stable
    activity over a large pH (2–13) and temperature range (40–100 ◦C). Various techniques, including:
    Diaoin, Octadecylsilane opened columns, and preparative HPLC coupled with testing bioactivity
    resulted in isolating a main active compound; this major inhibitor was identified as homogentisic acid
    (HGA). Notably, HGA was confirmed as a new inhibitor, a non-sugar-based aGI, and as possessing
    stronger activity than acarbose with IC50, and maximum inhibition values of 220 µg/mL, 95%, and
    1510 µg/mL, 65%, respectively. These results suggest that squid pens, an abundant and low-cost
    fishery processing by-product, constitute a viable source for the production of antidiabetic materials
    via fermentation by strains of Paenibacillus. This fermented product shows promising applications in
    diabetes or diabetes related to obesity treatment due to their stability, potent bioactivity, and efficient
    inhibition against mammalian enzymes.
    Relation: Marine Drugs, 15(9), p.274(11 pages)
    DOI: 10.3390/md15090274
    Appears in Collections:[Graduate Institute & Department of Chemistry] Journal Article

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