淡江大學機構典藏:Item 987654321/111828
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    Please use this identifier to cite or link to this item: https://tkuir.lib.tku.edu.tw/dspace/handle/987654321/111828


    Title: A nondestructive method to estimate the chlorophyll content of Arabidopsis seedlings
    Authors: Liang Ying, Daisuke Urano, Kang-Ling Liao, Tyson L Hedrick, Yajun Gao, Alan M Jones
    Keywords: Arabidopsis thaliana;C/N sensing;Chlorophyll content;ColorChecker chart;Heterotrimeric G protein complex;Stress assay
    Date: 2017-04-14
    Issue Date: 2017-10-25 02:10:56 (UTC+8)
    Abstract: Background
    Chlorophyll content decreases in plants under stress conditions, therefore it is used commonly as an indicator of plant health. Arabidopsis thaliana offers a convenient and fast way to test physiological phenotypes of mutations and treatments. However, chlorophyll measurements with conventional solvent extraction are not applicable to Arabidopsis leaves due to their small size, especially when grown on culture dishes.

    Results
    We provide a nondestructive method for chlorophyll measurement whereby the red, green and blue (RGB) values of a color leaf image is used to estimate the chlorophyll content from Arabidopsis leaves. The method accommodates different profiles of digital cameras by incorporating the ColorChecker chart to make the digital negative profiles, to adjust the white balance, and to calibrate the exposure rate differences caused by the environment so that this method is applicable in any environment. We chose an exponential function model to estimate chlorophyll content from the RGB values, and fitted the model parameters with physical measurements of chlorophyll contents. As proof of utility, this method was used to estimate chlorophyll content of G protein mutants grown on different sugar to nitrogen ratios.

    Conclusion
    This method is a simple, fast, inexpensive, and nondestructive estimation of chlorophyll content of Arabidopsis seedlings. This method lead to the discovery that G proteins are important in sensing the C/N balance to control chlorophyll content in Arabidopsis.
    Relation: Plant Methods. 2017; 13: 26.
    DOI: 10.1186/s13007-017-0174-6
    Appears in Collections:[Graduate Institute & Department of Mathematics] Journal Article

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