English  |  正體中文  |  简体中文  |  全文筆數/總筆數 : 59568/92818 (64%)
造訪人次 : 816369      線上人數 : 22
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library & TKU Library IR team.
搜尋範圍 查詢小技巧:
  • 您可在西文檢索詞彙前後加上"雙引號",以獲取較精準的檢索結果
  • 若欲以作者姓名搜尋,建議至進階搜尋限定作者欄位,可獲得較完整資料
  • 進階搜尋
    請使用永久網址來引用或連結此文件: http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/111828

    題名: A nondestructive method to estimate the chlorophyll content of Arabidopsis seedlings
    作者: Liang Ying, Daisuke Urano, Kang-Ling Liao, Tyson L Hedrick, Yajun Gao, Alan M Jones
    關鍵詞: Arabidopsis thaliana;C/N sensing;Chlorophyll content;ColorChecker chart;Heterotrimeric G protein complex;Stress assay
    日期: 2017-04-14
    上傳時間: 2017-10-25 02:10:56 (UTC+8)
    摘要: Background
    Chlorophyll content decreases in plants under stress conditions, therefore it is used commonly as an indicator of plant health. Arabidopsis thaliana offers a convenient and fast way to test physiological phenotypes of mutations and treatments. However, chlorophyll measurements with conventional solvent extraction are not applicable to Arabidopsis leaves due to their small size, especially when grown on culture dishes.

    We provide a nondestructive method for chlorophyll measurement whereby the red, green and blue (RGB) values of a color leaf image is used to estimate the chlorophyll content from Arabidopsis leaves. The method accommodates different profiles of digital cameras by incorporating the ColorChecker chart to make the digital negative profiles, to adjust the white balance, and to calibrate the exposure rate differences caused by the environment so that this method is applicable in any environment. We chose an exponential function model to estimate chlorophyll content from the RGB values, and fitted the model parameters with physical measurements of chlorophyll contents. As proof of utility, this method was used to estimate chlorophyll content of G protein mutants grown on different sugar to nitrogen ratios.

    This method is a simple, fast, inexpensive, and nondestructive estimation of chlorophyll content of Arabidopsis seedlings. This method lead to the discovery that G proteins are important in sensing the C/N balance to control chlorophyll content in Arabidopsis.
    關聯: Plant Methods. 2017; 13: 26.
    DOI: 10.1186/s13007-017-0174-6
    顯示於類別:[數學學系暨研究所] 期刊論文


    檔案 描述 大小格式瀏覽次數
    A nondestructive method to estimate the chlorophyll content of Arabidopsis seedlings.pdf1746KbAdobe PDF0檢視/開啟



    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library & TKU Library IR teams. Copyright ©   - 回饋