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    Please use this identifier to cite or link to this item: http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/109597


    Title: Perturbation of cytosolic calcium by 2-aminoethoxydiphenyl borate and caffeine affects zebrafish myofibril alignment
    Authors: Wu, Hsin-Ju Wu;Fong, Tsorng-Harn;Chen, Shen-Liang;Wei, Jen-Cheng;Wang, I-Jong;Wen, Chi-Chung;Chang, Chao-Yuan;Chen, Xing-Guang;Chen, Wei-Yu;Chen, Hui-Min;Horng, Juin-Lin;Wang, Yun-Hsin;Chen, Yau-Hung
    Date: 2015-03-01
    Issue Date: 2017-02-24 02:12:20 (UTC+8)
    Publisher: John Wiley & Sons Ltd.
    Abstract: The objective of the current study was to investigate the effects of Ca2+ levels on myofibril alignment during zebrafish embryogenesis. To investigate how altered cytoplasmic Ca2+ levels affect myofibril alignment, we exposed zebrafish embryos to 2-aminothoxyldiphenyl borate (2-APB; an inositol 1,4,5-trisphosphate receptor inhibitor that reduces cytosolic Ca2+ levels) and caffeine (a ryanodine receptor activator that enhances cytosolic Ca2+ levels). The results demonstrated that the most evident changes in zebrafish embryos treated with 2-APB were shorter body length, curved trunk and malformed somite boundary. In contrast, such malformed phenotypes were evident neither in untreated controls nor in caffeine-treated embryos. Subtle morphological changes, including changes in muscle fibers, F-actin and ultrastructures were easily observed by staining with specific monoclonal antibodies (F59 and α-laminin), fluorescent probes (phalloidin) and by transmission electron microscopy. Our data suggested that: (1) the exposure to 2-APB and/or caffeine led to myofibril misalignment; (2) 2-APB-treated embryos displayed split and short myofibril phenotypes, whereas muscle fibers from caffeine-treated embryos were twisted and wavy; and (3) zebrafish embryos co-exposed to 2-APB and caffeine resulted in normal myofibril alignment. In conclusion, we proposed that cytosolic Ca2+ is important for myogenesis, particularly for myofibril alignment.
    Relation: Journal of Applied Toxicology 35(3), pp.287-294
    DOI: 10.1002/jat.3057
    Appears in Collections:[化學學系暨研究所] 期刊論文

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