The present paper proposes a method to directly measure pressure distribution in a microchannel. A polyvinylidene fluoride (PVDF) piezoelectric film was packaged on the inside wall of a microchannel and connected to an external signal filtering amplifier to the oscilloscope to observe the voltage response when the flow through the microchannel and then the voltage response of the PVDF film converted into the pressure response. The experimental results demonstrated that the obtained data were quite consistent with the numerical calculations, and the pressure distribution in the microchannel presents a nonlinear function in which pressure increased with decreasing distance from the entrance.