Measurement of in-plane elasticity of live cell layers using a pressure sensor embedded microfluidic device

doi:10.1038/srep36425

機構典藏 > College of Engineering > Graduate Institute & Department of Civil Engineering > Journal Article > Item 987654321/108581

Please use this identifier to cite or link to this item: https://tkuir.lib.tku.edu.tw/dspace/handle/987654321/108581

Title:	Measurement of in-plane elasticity of live cell layers using a pressure sensor embedded microfluidic device
Authors:	Lin, Chien-Han;Wang, Chien-Kai;Chen, Yu-An;Peng, Chien-Chun;Liao, Wei-Hao;Tung, Yi-Chu
Keywords:	Biomedical engineering;Lab-on-a-chip
Date:	2016-11-04
Issue Date:	2016-11-30 02:11:04 (UTC+8)
Publisher:	Nature Publishing Group
Abstract:	In various physiological activities, cells experience stresses along their in-plane direction when facing substrate deformation. Capability of continuous monitoring elasticity of live cell layers during a period is highly desired to investigate cell property variation during various transformations under normal or disease states. This paper reports time-lapsed measurement of live cell layer in-plane elasticity using a pressure sensor embedded microfluidic device. The sensor converts pressure-induced deformation of a flexible membrane to electrical signals. When cells are cultured on top of the membrane, flexural rigidity of the composite membrane increases and further changes the output electrical signals. In the experiments, human embryonic lung fibroblast (MRC-5) cells are cultured and analyzed to estimate the in-plane elasticity. In addition, the cells are treated with a growth factor to simulate lung fibrosis to study the effects of cell transformation on the elasticity variation. For comparison, elasticity measurement on the cells by atomic force microscopy (AFM) is also performed. The experimental results confirm highly anisotropic configuration and material properties of cells. Furthermore, the in-plane elasticity can be monitored during the cell transformation after the growth factor stimulation. Consequently, the developed microfluidic device provides a powerful tool to study physical properties of cells for fundamental biophysics and biomedical researches.
Relation:	Scientific Reports 6, p.36425(14 pages)
DOI:	10.1038/srep36425
Appears in Collections:	[Graduate Institute & Department of Civil Engineering] Journal Article

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