Application of chitinous materials in production and purification of a poly(L-lactic acid) depolymerase from Pseudomonas tamsuii TKU015

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题名:	Application of chitinous materials in production and purification of a poly(L-lactic acid) depolymerase from Pseudomonas tamsuii TKU015
作者:	Tzu-Wen Liang;Shan-Ni Jen;Anh Dzung Nguyen;San-Lang Wang
关键词:	PLA;squid pen;Pseudomonas tamsuii;PLA depolymerase;degradation
日期:	2016-03-01
上传时间:	2016-08-15
出版者:	MDPIAG
摘要:	The management of fishery residues and plastics is considered to be a vital strategy for conserving resources and maintaining the quality of the environment. Poly(l-lactic acid) (PLA) is a commercially promising, renewable, and biodegradable plastic. In this study, a PLA depolymerase was produced in a squid pen powder (SPP) and recycled plastic waste (PLA powder)-containing medium by Pseudomonas tamsuii TKU015, a bacterial strain isolated from Taiwanese soil. This PLA depolymerase had a molecular weight of 58 kDa and was purified to homogeneity from the supernatant of a TKU015 culture. The optimum pH of TKU015 PLA depolymerase is 10, and the optimal temperature of the enzyme is 60 °C. In addition to PLA, TKU015 PLA depolymerase degraded fibrinogen and tributyrin, but did not hydrolyze casein, triolein, and poly(β-hydroxybutyrate). Taken together, these data demonstrate that P. tamsuii TKU015 produces a PLA depolymerase to utilize SPP and polylactide as carbon/nitrogen sources.
關聯:	Polymers 8(3), pp.98(11 pages)
DOI:	10.3390/polym8030098
显示于类别:	[化學學系暨研究所] 期刊論文

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