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    Please use this identifier to cite or link to this item: http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/107110

    Title: Application of chitinous materials in production and purification of a poly(L-lactic acid) depolymerase from Pseudomonas tamsuii TKU015
    Authors: Tzu-Wen Liang;Shan-Ni Jen;Anh Dzung Nguyen;San-Lang Wang
    Keywords: PLA;squid pen;Pseudomonas tamsuii;PLA depolymerase;degradation
    Date: 2016/03/01
    Issue Date: 2016-08-15
    Publisher: MDPIAG
    Abstract: The management of fishery residues and plastics is considered to be a vital strategy for conserving resources and maintaining the quality of the environment. Poly(l-lactic acid) (PLA) is a commercially promising, renewable, and biodegradable plastic. In this study, a PLA depolymerase was produced in a squid pen powder (SPP) and recycled plastic waste (PLA powder)-containing medium by Pseudomonas tamsuii TKU015, a bacterial strain isolated from Taiwanese soil. This PLA depolymerase had a molecular weight of 58 kDa and was purified to homogeneity from the supernatant of a TKU015 culture. The optimum pH of TKU015 PLA depolymerase is 10, and the optimal temperature of the enzyme is 60 °C. In addition to PLA, TKU015 PLA depolymerase degraded fibrinogen and tributyrin, but did not hydrolyze casein, triolein, and poly(β-hydroxybutyrate). Taken together, these data demonstrate that P. tamsuii TKU015 produces a PLA depolymerase to utilize SPP and polylactide as carbon/nitrogen sources.
    Relation: Polymers 8(3), pp.98(11 pages)
    DOI: 10.3390/polym8030098
    Appears in Collections:[化學學系暨研究所] 期刊論文

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