淡江大學機構典藏:Item 987654321/106198
English  |  正體中文  |  简体中文  |  全文笔数/总笔数 : 56831/90538 (63%)
造访人次 : 12258479      在线人数 : 59
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library & TKU Library IR team.
搜寻范围 查询小技巧:
  • 您可在西文检索词汇前后加上"双引号",以获取较精准的检索结果
  • 若欲以作者姓名搜寻,建议至进阶搜寻限定作者字段,可获得较完整数据
  • 进阶搜寻


    jsp.display-item.identifier=請使用永久網址來引用或連結此文件: http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/106198


    题名: Detecting trypsin at liquid crystal/aqueous interface by using surface-immobilized bovine serum albumin
    作者: Cheng-Hao Chuang;Yi-Cheng Lin;You-Shuan Chen;Yu-Xun Chen;Chieh-Ming Chen;Hung Wei Shiu;Lo-Yueh Chang;Chia-Hao Chen;Chih-Hsin Chen
    关键词: Liquid crystal-based biosensor;Bovine serum albumin;Trypsin;Scanning photoelectron microscopy
    日期: 2016-04-15
    上传时间: 2016-04-22 13:24:06 (UTC+8)
    出版者: Elsevier
    摘要: We report a new mechanism for liquid crystal (LC)-based sensor system for trypsin detection. In this system, bovine serum albumin (BSA) was immobilized on gold grids as the enzymatic substrate. When the BSA-modified grid was filled with LC and immersed in the solution containing trypsin, the peptide bonds of BSA were hydrolyzed and peptide fragments were desorbed from the surface of gold grid, which disrupted the orientation of LC at the vicinity and resulted in a dark-to-bright transition of optical image of LCs. By using this mechanism, the limit of detection (LOD) of trypsin is 10 ng/mL, and it does not respond to thrombin and pepsin. Besides, the cleavage behavior on gold surfaces was directly visualized by the scanning photoelectron microscopy (SPEM), in particular for the chemical composition identification and element-resolved image. The loss of BSA fragments and the enhancement of Au photoelectron signal after trypsin cleavage were corresponding to the proposed mechanism of the LC-based sensor system. Because the signals reported by LC can be simply interpreted through the human naked-eye, it provides a simple method for fast-screening trypsin activity in aqueous solution.
    關聯: Biosensors and Bioelectronics 78, p.213–220
    DOI: 10.1016/j.bios.2015.11.049
    显示于类别:[物理學系暨研究所] 期刊論文
    [化學學系暨研究所] 期刊論文

    文件中的档案:

    档案 描述 大小格式浏览次数
    Detecting trypsin at liquid crystal_aqueous interface by using surface-immobilized bovine serum albumin.pdf4506KbAdobe PDF0检视/开启
    index.html0KbHTML253检视/开启

    在機構典藏中所有的数据项都受到原著作权保护.

    TAIR相关文章

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library & TKU Library IR teams. Copyright ©   - 回馈