工作壓力是近年受到組織行為學者大量關注之議題，另一方面，許多企業開始重視員工幸福感對提昇組織績效的重要性，並思索如何透過員工在性格上的差異，減輕員工的工作壓力，提昇員工的主觀幸福感。故本研究欲檢驗A型性格對工作壓力源與與主觀幸福感的影響，同時檢驗工作壓力源對主觀幸福感的影響。本研究利用網路問卷，蒐集328份有效問卷後，進行後續分析。層級迴歸分析結果發現，A型性格對工作壓力有正向影響，工作壓力源對對主觀幸福感有顯著負向影響。此外，A型性格對主觀幸福感則無顯著影響。最後，本研究根據上述結果，提出相關理論與實務意涵建議。 The scholars in the field of organizational behavior have paid increasing attention on work stress in the past decades. Organizations have also emphasized the importance of well-being to the improvement of organizational performance, and have attempted to influence work stress and well-being via employees’ personality traits. The purposes of the study are to examine the influence of Type-A personality on work stress and well-being as well as the influence of work stress on well-being. We collected 328 copies of effective questionnaires via online survey, and then conducted subsequent analyses. The results of hierarchical regression analysis showed that Type-A personality had positive effects on work stress, and work stress had negative effects on well-being. In addition, Type-A personality had no significant effect on well-being. Both theoretical and practical implications are discussed.