本研究主要分為兩個部分。第一部分是利用電激發化學發光法( ECL, Electrogenerated chemiluminescence )搭載自動化流注系統( flow injection analysis, FIA )偵測糖尿病用藥米格列醇，主要是利用電化學的方式使Ru(bpy)32+在電極表面氧化產生Ru(bpy)33+並催化三級胺類米格列醇使米格列醇形成自由基，米格列醇自由基再與Ru(bpy)33+反應形成激發態Ru(bpy)32+＊而其由激發態返回基態時放出冷光(λ=620nm)以偵測冷光的方式達到定量米格列醇的目的，此FIA-ECL偵測系統在最佳化的條件下線性範圍為0.1 μM ~ 10 μM (R2=0.999)，偵測極限(S/N=3)為55.3 nM，更大的線性範圍可達15 μM (R2=0.998)，偵測極限(S/N=3)為58.11 nM，相對標準偏差(RSD)為0.52%。第二部分則是將此系統應用於高效液相層析儀( HPLC )上，將干擾物與米格列醇分離以達到在尿液生化樣品上分析應用的目的其線性範圍為1 μM~30 μM，偵測極限(S/N=3)為0.78 μM，此分析特性也符合在臨床上檢測之使用。 There are two parts in this study. The first one, determination of miglitol with Electrogenerated chemiluminescence ( ECL ) with flow injection analysis ( FIA ) was fabricated. In electrode surface Ru(bpy)32+ go through electron-transfer reaction with miglitol (co-reactant) to form excited state Ru(bpy)32+＊, and excited state Ru(bpy)32+＊ back to the ground state that emit light by vibrational energy relaxation( VR ). In this analysis system, the linear range of miglitol is between 0.1 μM and 10 μM (R2=0.999), sensitivity is 570.5 /μM, detection limit (S/N=3) is 58.11 nM, and relative standard deviation of twenty repetitive detection is 0.52%. The second one, we combine ECL with HPLC to seprate interference in urine samples. HPLC is used to separate miglitol and matrix in urine samples and determination of miglitol by PMT. The linear range in HPLC-ECL system is between 1 μM and 30 μM, detection limit (S/N=3) is 0.78 μM.
