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    题名: Production and purification of a fungal chitosanase and chitooligomers from Penicillium janthinellum D4 and discovery of the enzyme activators.
    作者: Nguyen, Anh Dzung;Huang, Chun-Ching;Liang, Tzu-Wen;Nguyen, Van Bon;Pan, Po-Shen;Wang, San-Lang
    关键词: Boron;Chitooligomers;Chitosanase;Chitosanase activator;Penicillium janthinellum;Squid pen
    日期: 2014-08-08
    上传时间: 2016-01-06 11:06:46 (UTC+8)
    摘要: Chitosanases have received much attention because of their wide range of applications. Although most fungal chitosanases use sugar as their major carbon source, in the present work, a chitosanase was induced from a squid pen powder (SPP)-containing Penicillium janthinellum D4 medium and purified by ammonium sulphate precipitation and combined column chromatography. The purified D4 chitosanase exhibited optimum activity at pH 7-9, 60°C and was stable at pH 7-11, 25-50°C. The D4 chitosanase that was used for chitooligomers preparation was studied. The enzyme products revealed various chitooligomers with different degrees of polymerisation (DP) from 3 to 9, as determined by a MALDI-TOF mass spectrometer, confirming the endo-type nature of the D4 chitosanase. D4 chitosanase activity was significantly inhibited by Cu(2+), Mn(2+), and EDTA. However, Fe(2+) activated or inhibited D4 chitosanases at different concentrations. The D4 chitosanase was also activated by some small synthetic boron-containing molecules with boronate ester side chains.
    關聯: Carbohydrate Polymers 108, pp.331-337
    DOI: 10.1016/j.carbpol.2014.02.053.
    显示于类别:[化學學系暨研究所] 期刊論文

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