本研究主要探討利用乾噴溼紡法製備PCL中空纖維管薄膜，以界面活性劑Tween20作為成孔劑，使用異丙醇水溶液作為軟性沉澱槽，同時探討芯液與沉澱液中異丙醇濃度對薄膜結構的影響，並於內表面塗佈幾丁聚醣，探討添加幾丁聚醣對許旺細胞增生之影響。藉由SEM、DSC、ATR、拉力測試、接觸角量測及酵素分解測試等來分析中空纖維管薄膜的結構與性質；最後利用SEM、MTT與LDH分析，比較各薄膜對許旺細胞培養之優劣性。 以不同異丙醇水溶液濃度的芯液與沉澱液來製備中空纖維管薄膜時，由SEM圖觀測到薄膜的結構隨著芯液的軟性程度越高，內表面孔洞逐漸增加，芯液異丙醇濃度達到15 %(v/v)時，薄膜內表面有較為明顯的孔隙出現，孔徑為10至15um；當沉澱液軟性程度越高，內表面孔徑提升得越明顯，更甚者會形成網狀結構孔洞。而在ATR的分析中，得知幾丁聚醣於塗佈程序下確實會在內表面沉積。DSC的檢測則發現，塗佈幾丁聚醣薄膜會有異質成核現象，造成冷結晶溫度的上升。拉力測試則顯示機械性質與整體薄膜巨孔結構含量有關，塗佈幾丁聚醣於表面可提升薄膜斷裂拉伸應力，但幾丁聚醣填入薄膜內部時將快速降低整體機械性質。細胞增生與死亡率顯示，緻密皮層結構能提供良好的初期細胞表面貼附，內層塗佈幾丁聚醣下對細胞成長有一定程度的提升。以Pseudomonas cepacia Lipase作為分解酵素可於第5至6天下將薄膜分解完全。 Poly(-caprolactone) hollow fiber was prepared by wet spinning method for the guidance on the proliferation of Schwann cells. The PCL was dissolved in DMAc as solvent incorporated with tween20 as a pore former to modify the structure and the isopropanol solution was used as a soft coagulant. The morphology of the membrane could be affected by increasing the concentration of IPA solution of the bore liquid and the coagulant bath. The PCL hollow fiber was coated with chitosan solution to improve the proliferation of Schwann cells. Scanning electron microscope (SEM), Differential Scanning Calorimetry (DSC), Fourier Transform Infrared-Attenuated total reflection (FTIR-ATR), tensile mechanical properties, contact angle and enzyme degradation were used to characterize the structure and properties of membrane. On the other hand, the effect of proliferation of Schwann cells was analyzed by SEM, MTT assay and LDH assay. The effects of IPA solution concentration in coagulant and bore liquids on the morphologies were studied by SEM. The results showed that pore size of the inner surface increased when the bore liquid become more and more soft. When we used the 15 %(v/v) IPA solution as the bore liquid, the pore size is about 10 to 15 um. The pore size and porosity of the inner surface increased to net structure when IPA concentration of the the coagulation was increased. The results of Fourier Transform Infrared-Attenuated total reflection (FTIR-ATR) showed that chitosan solution could directly coated on the inner surface of hollow fiber. The tensile mechanical properties were dependant on the macrovoid structure. The tensile break strength of membranes whose inner surface was treated with chitosan solution was higher; however, the break stength was lowered when the chitosan diffused inside the membrane. The results of Schwann cell culture showed that the hollow fiber treated with chitosan had better cell adhesion behavior than the untreated one. The results of enzyme degradation using the Pseudomonas cepacia Lipase could completely degrade hollow fiber in 5 to 6 days.
