Liquid crystal-based immunoassay for detecting human serum albumin

淡江大學機構典藏 > 理學院 > 化學學系暨研究所 > 期刊論文 > Item 987654321/100078

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題名:	Liquid crystal-based immunoassay for detecting human serum albumin
作者:	Chen, Chih-Hsin;Ho, Wen-Hsun
貢獻者:	淡江大學化學學系
日期:	2014-03-27
上傳時間:	2015-01-28 11:05:08 (UTC+8)
出版者:	Dordrecht: Springer Netherlands
摘要:	Human serum albumin (HSA) is the most abundant protein in human blood plasma. It is commonly used as a biomarker in urine samples to identify the chronic kidney disease caused by high blood pressure or diabetes. In our research, a thin layer of liquid crystals (LCs) is used as a readout system for developing an immunoassay that reports the presence of HSA in the aqueous solution with optical signals. The detection principle of this assay is based on the variation of surface density of protein upon the specific binding of HSA on an anti-HSA immobilized surface, which leads a dark-to-bright transition of LC images under cross-polarizers. Our results show that the LC-based immunoassay can detect HSA at a concentration of 50 μg/mL. By using the slide with immobilized anti-HSA in array format, the concentration level of HSA can be simply determined by the number of LC spot shown on the slide.
關聯:	Research on Chemical Intermediates 40(6), pp.2229-2236
DOI:	10.1007/s11164-014-1600-2
顯示於類別:	[化學學系暨研究所] 期刊論文

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