DSpace collection: 專書之單篇

Thermodynamics and Kinetics of Protein Folding and Aggregation

Thu, 06 Jun 2019 04:10:29 GMT

title: Thermodynamics and Kinetics of Protein Folding and Aggregation abstract: Thermodynamic and kinetic properties of proteins affect their folding and aggregation processes, which are responsible for a range of biological functions in cells. This chapter aims to introduce simple models of protein folding and aggregation, the nucleation–growth kinetics of protein aggregation, and its study in thermodynamic interpretation of aggregation mechanism. We first review the two-state thermodynamics of protein folding and a generalized two-state scheme, i.e., Ising model. Several Ising-based protein models are discussed, including a generalized Ising model for studying mean-field theory of protein folding and a modified Wako-Saitô-Muñoz-Eaton (M-WSME) model for describing foldon behavior of protein. Also, we review some mechanisms of protein aggregation and a statistical mechanical approach in addressing aggregation equilibrium properties. Finally, we report our recent work on the kinetics of protein aggregation using a kinetic Ising model and discuss diverse aggregation scenarios through a kinetic survey over four different amyloid proteins.

回顧日本科技發展五大階段

Sat, 03 Nov 2012 06:18:21 GMT

title: 回顧日本科技發展五大階段

儀器分析：高效能液相層析、氣相層析分析

Fri, 30 Sep 2011 13:03:16 GMT

title: 儀器分析：高效能液相層析、氣相層析分析 description: 儀器分析第11章、第12章

提昇女性的科技參與

Tue, 01 Dec 2009 01:01:16 GMT

title: 提昇女性的科技參與

DNA Capillary Electrophoresis Using Block Copolymer as a New Separation Medium

Tue, 01 Dec 2009 01:01:14 GMT

title: DNA Capillary Electrophoresis Using Block Copolymer as a New Separation Medium abstract: The development of automated DNA sequencing has drawn the attention of many research groups in recent years, especially for the high-throughput demand of the Human Genome Project (1,2). Automation of DNA sequencing based on standard electrophoresis is the most commonly accepted and used approach. Capillary electrophoresis (CE) using replaceable polymer solution as a rapid separation medium with high-resolution separations can be automated relatively easily when compared with slab-gel electrophoresis. The development of capillary array electrophoresis (CAE) has further enhanced the throughput of DNA sequencing. The application of replaceable polymer solutions over permanent crosslinked gels as a separation medium made CE the best conventional analytical method at this time (3)f because gels tend to be more difficult to handle and have limited column lifetime.

Amphiphilic Polyoxyalkylene Triblock Copolymers: Self-Assembly, Phase Behaviors, and New Applications

Tue, 01 Dec 2009 01:01:11 GMT

title: Amphiphilic Polyoxyalkylene Triblock Copolymers: Self-Assembly, Phase Behaviors, and New Applications abstract: The study on the micellization behavior of amphiphilic block copolymers in aqueous media has reached the stage where semi-quantitative analysis and prediction of micellar parameters and phase behavior are possible. The deeper understanding has provided an incentive to explore new applications on these self-assembled systems. In this chapter, we summarize the self-assembly and phase behavior of polyoxyalkylene triblock copolymers containing E and P, or E and B blocks (with E, P, B being polyoxyethylene, polyoxypropylene and polyoxybutylene, respectively), in aqueous solution. Laser light scattering was used to characterize micellar parameters in the dilute solution regime and small-angle X-ray scattering was employed to study gel-like ordered packings of the micelles at high polymer concentrations. The hydrophobic blocks form micellar cores and are mainly responsible for the formation of micelles (cmc, aggregation number, and thermodynamic parameters). The hydrophilic block length determines the micellar size and intermicellar interactions. The co-association behavior of a mixture of two different block copolymers in aqueous solution has also been elucidated. The effect of block length on phase separation (clouding temperature) can be discussed on the basis of experimentally determined empirical rules. At high polymer concentrations, unique gel-like micellar packings and their spatial supramolecular rearrangements (e.g., bcc, hexagonal, lamellar) occurred. The phase diagrams could be very rich, especially with the addition of another immiscible solvent. These gel-like materials with ordered hydrophilic-hydrophobic packings gave access to new applications in analytical and biological sciences — the structure served as a medium in capillary gel electrophoresis, e.g., for double-stranded DNA analysis with single base-pair resolution.