DSpace collection: 研究報告

Pro-angiogenic effects of chalcone derivatives in zebrafish embryos in vivo

title: Pro-angiogenic effects of chalcone derivatives in zebrafish embryos in vivo

Nephroprotective role of resveratrol and ursolic acid in aristolochic acid intoxicated zebrafish

title: Nephroprotective role of resveratrol and ursolic acid in aristolochic acid intoxicated zebrafish

Knocking down 10-formyltetrahydrofolate dehydrogenase increased oxidative stress and impeded zebrafish embryogenesis by obstructing morphogenetic movement

title: Knocking down 10-formyltetrahydrofolate dehydrogenase increased oxidative stress and impeded zebrafish embryogenesis by obstructing morphogenetic movement

Mechanism for controlling the monomer-dimer conversion of SARS coronavirus main protease

title: Mechanism for controlling the monomer-dimer conversion of SARS coronavirus main protease

Inactivation of myosin binding protein C homolog in zebrafish as a model for human cardiac hypertrophy and diastolic dysfunction

title: Inactivation of myosin binding protein C homolog in zebrafish as a model for human cardiac hypertrophy and diastolic dysfunction

幾丁質類物質經Serratia屬細菌發酵生產生理活性化合物之分離及鑑定

title: 幾丁質類物質經Serratia屬細菌發酵生產生理活性化合物之分離及鑑定 abstract: 本研究擬分三年時間, 以Serratia ureilytica TKU013所生產生理活性成分為主，及實驗室已篩選到的其他Serratia屬細菌，分別比較其發酵上清液之生理活性及結構鑑定。所擬探討的碳氮源包括蝦殼、蟹殼、烏賊軟骨以及其他各種幾丁質及幾丁聚醣產品（不同去乙醯度、分子量）。『第一年』擬針對Serratia spp.所生產抗氧化劑進行其最適生產條件之探討，並分析抗氧化活性之機制及其性質之研究，再比較Serratia ureilytica TKU013與其他 Serratia spp.菌株之發酵上清液其抗氧化活性之差異。『第二年』擬探討抗氧化物成分之萃取、分離及化學結構之鑑定，並將純化後之成份分別進行抗氧化及抗腫瘤評估。『第三年』擬探討有效成份之抗腫瘤機轉分析及有效成份大量生產方法之評估。此類研究成果得以應用於諸如保健食品及化妝品之類的生物技術或生物醫學材料方面之用途。
description: 計畫編號：NSC99-2313-B032-001-MY3

野外菌株中 Liver X Receptor 活化物之鑑定

title: 野外菌株中 Liver X Receptor 活化物之鑑定 description: 研究期間：20100701~20100930

幾丁質類物質經Serratia屬細菌發酵生產生理活性化合物之分離及鑑定

轉錄因子NF-Y, Capsulin與Musculin參與顏面肌肉軟骨發育之分子機制探討

淡江大學基礎科學人才培育銜接計畫─斑馬魚之X光成相研究

title: 淡江大學基礎科學人才培育銜接計畫─斑馬魚之X光成相研究 description: 研究期間：20070201~20071231

日本保健生物科技產品之資訊收集

title: 日本保健生物科技產品之資訊收集 description: 研究期間：20060501~20060731

中小企業與淡江大學生命科學開發中心合作計畫

title: 中小企業與淡江大學生命科學開發中心合作計畫 description: 研究期間：20040301~20040930

含膳食纖維耐保存之乳酸菌天然機能食品之開發

title: 含膳食纖維耐保存之乳酸菌天然機能食品之開發 description: 研究期間：20040101~20040630

幾丁類物質於生產細菌幾丁質分解酵素及寡醣製備之應用

title: 幾丁類物質於生產細菌幾丁質分解酵素及寡醣製備之應用 abstract: 本研究擬分三年時間, 以B. amyloliquefaciens V656 所生產幾丁質.為主，配合實驗室已篩選到四株新穎幾丁質.及/或溶菌.及/或蛋白.生產菌（B. subtilis W-118, B. subtilis TKU007, Bacillus sp. TKU004, Pseudomonas aeruginosa K-187）作為酵素來源。所擬探討的基質（酵素水解之對象）包括蝦殼、蟹殼、烏賊軟骨、紅麴菌絲以及其他各種幾丁質及幾丁聚醣產品（不同去乙醯度、分子量）。『第一年』擬藉由HPLC來探討上述細菌所生產酵素（幾丁質.及/或溶菌.及 /或蛋白.）水解各種基質生產幾丁寡醣（以幾丁六醣及/或七醣為主要目標）之較適條件，並將所得水解產物進行抗腫瘤測試。『第二年』擬藉由AS-L這種酸鹼可逆型擔體進行酵素固定化，探討利用固定化酵素連續生產較多量幾丁寡醣之可行性，並將所得水解產物進行抗腫瘤測試。『第三年』擬探討這些水解物之免疫活性，以及分析所具抗腫瘤之機轉，探討所得各種水解產物於抗癌及生醫材料研發之應用潛力。 This is a tree years project. Five bacterial strains , Bacillus amyloliquefaciens V656, B. subtilisW-118, B. subtilis TKU007, Bacillus sp. TKU004, and Pseudomonas aeruginosa K-187 were used for the production of novel chitinases and/or proteases. The chitinous materials used as the enzymatic substrates for preparation of chitooligosaccharides were shrimp shells, squid pen, chitin, chitosan, and cell walls Monascus hyphae. In the first year, the reaction conditions of enzymatic hydrolysis were studied. The produced chitooligosaccharides were analyzed by HPLC. The antitumor activity of these chitooligosaccharides containing hydrolyzates will be analyzed. In the second year, a reversibly soluble polymer (AS-L) will be used as the carrier to immobilized the enzymes produced by above five bacteria strains. The immobilized enzymes will be used to produced large amount of chitooligosaccharides. In the third year, the mechanism of chitooligosaccharides to tumor cells will be study in detail.
description: 計畫編號：NSC96-2313-B032-002-MY3

幾丁類物質於生產細菌幾丁質分解酵素及寡醣製備之應用

轉錄因子NF-Y, Capsulin與Musculin參與顏面肌肉軟骨發育之分子機制探討

title: 轉錄因子NF-Y, Capsulin與Musculin參與顏面肌肉軟骨發育之分子機制探討 abstract: 頭部肌肉與軟骨的發育起源於神經脊細胞，頭部的神經脊細胞來自於後腦，然後經移動而進入咽弧，最後再與來自中胚層的間葉細胞以及周圍的上皮細胞間進行交互作用，再經由某些未知基因的作用之下而形成頭部肌肉與骨骼的前驅細胞。頭部的肌肉前驅細胞接著會受到MRF 基因群的調控進而形成肌肉；頭部的骨骼前驅細胞接著會受到sox9a、runx2 與cbfa 等基因群的調控進而形成骨骼。本期計畫欲研究之capsulin、musculin 與nf-y 等基因群，據推測，應該在神經脊細胞與頭部肌肉與骨骼的前驅細胞之間，扮演重要的角色(Fig. 9)。這些研究項目十分繁重，故擬分三年來完成下列目標。第一年計畫內容涵蓋：(1)複殖出capsulin、musculin 與nf-y 等基因之cDNA 全長；(2)進行全個體雜交實驗，以便偵測capsulin、musculin 與nf-y 等基因在胚胎發育早期的表現位置；(3)切片技術與組織染色；(4) capsulin、musculin 與nf-y 等基因結構之確定；(5)抗體免疫螢光染色實驗的建立。第二年計畫內容涵蓋：(1)胚胎發育抑制劑的注射；(2)切片技術與組織染色； (3)抗體免疫螢光染色實驗的建立；(4)軟骨染色；(5)細胞凋亡實驗之建立。第三年計畫內容涵蓋：(1)胚胎發育抑制劑的注射；(2)全長capsulin、 musculin、nf-y、myf5、myod 之mRNA 合成與注射；(3)軟骨染色；(4)抗體免疫螢光染色實驗的建立；(5)切片技術與組織染色；(6) capsulin、musculin 與nf-y 參與頭部肌肉軟骨發育機制之探討。 Cranial neural crest (CNC) cells play an important role during cranial myogenesis and cartilage development. CNC cell originates from hindbrain, migrates into pharyngeal arch, and then differentiates into muscle and cartilage under the control of some unknown genes. We hypothesize that capsulin, musculin and nf-y genes are upstream regulators that converting CNC into muscle and cartilage cell fates (Fig. 9). To confirm this hypothesis, we propose this 3-year project. Our proposal will be including: The first year: (1). Cloning the full length cDNA of capsulin, musculin and nf-y; (2). Carrying out whole mount in situ hybridization experiments to detect the endogenous expression patterns of capsulin, musculin and nf-y during early embryogenesis; (3). Using cryosection and immunohistochemical staining to further confirm the expression domain of each gene transcript; (4). Determination the genomic organizations of capsulin, musculin and nf-y genes; (5). Using antibodies staining to study the endogenous protein levels. The second year: (1). Morpholino injection to knockdown capsulin, musculin and nf-y; (2). Using cryosection and immunohistochemical staining to further confirm the expression domain of each gene transcript; (3). Using antibodies staining to study the endogenous protein levels; (4). Using alician blue staining to visualize the cartilages; (5). Carrying out TUNEL assay to detect the apoptotic cells. The third year: (1). Morpholino injection to knockdown capsulin, musculin and nf-y; (2). Synthesis of full length capsulin, musculin and nf-y mRNA and co-injection them with morpholino into zebrafish embryos; (3). Using alician blue staining to visualize the cartilages; (4). Using antibodies staining to study the endogenous protein levels; (5). Using cryosection and immunohistochemical staining to further confirm the expression domain of each gene transcript; (6) Discovery the molecular mechanisms of how capsulin, musculin and nf-y are involved during craniofacial development.
description: 計畫編號：NSC97-2313-B032-001-MY3

開發基底細胞癌之斑馬魚品系用以篩選新型釕金屬錯合物之抗腫瘤前導藥物

title: 開發基底細胞癌之斑馬魚品系用以篩選新型釕金屬錯合物之抗腫瘤前導藥物 abstract: 抗癌藥物的篩選如果有適當又便宜的疾病動物模式可使用，可降低成本，大大提升效率。斑馬魚在基因轉殖以及穩定遺傳方面的技術已經成熟，儼然成為研究癌症發生學不可或缺的利器。所以擬研提今年這個研究計畫，也就是應用基因轉殖配合穩定遺傳的技術，在表皮細胞過度表現shh 與gli2 等致癌蛋白並且與紅螢光蛋白(RFP)報導基因融合，期望得到穩定遺傳、會發紅螢光而且分別會罹患先天性基底細胞癌與自發性基底細胞癌轉殖品系。最後利用這些轉殖品係來篩選本校化學系所合成的小分子化學物資料庫(尤其是新型釕金屬錯合物)，希望篩選出抗皮膚癌之新型前導藥物藥。這些研究項目十分繁重，故擬分三年來完成下列目標。第一年計畫內容涵蓋：(1)構築shh 與gli 等致癌蛋白並且與紅螢光蛋白(RFP) 報導基因融合的表現質體；(2)顯微注射法將上述質體植入斑馬魚體內；(3)穩定遺傳品系的獲得；(4)切片技術與組織染色；(5)轉殖品系的量產。第二年計畫內容涵蓋：(1) 轉殖品系的量產；(2) 切片技術與組織染色；(3) 抗體免疫螢光染色實驗的建立；(4) Microarray 的分析；(5) 先天性基底細胞癌與自發性基底細胞癌的細胞癌化分子機制探討。第三年計畫內容涵蓋：(1) 轉殖品系的量產；(2) 釕金屬錯合物的篩選；(3) 藥物處理後的動態螢光觀察；(4)抗體免疫螢光染色實驗的建立；(5)切片技術與組織染色；(6)其他抗癌前導藥物的篩選。 Screening of the anti-tumor drugs is very laborious and expensive. The efficiencies will be greatly improved and the cost will be reduced if there is a suitable animal model ready to use. The transgenic and germ-line transmission techniques are mature enough to make it possible that using zebrafish as an animal model to study human cancer and diseases. We will create two zebrafish lines which are overexpression shh and gli oncoproteins on their skin and are ready for mimicking autosomal dominantly basal cell carcinoma syndrome and sporadic basal cell carcinoma, respectively, to screen anti-skin tumor leading-drugs in vivo in this 3-year project. Our proposal will be including: The first year: (1). Construction of the shh and gli fusing with RFP, and are driven by zebrafish keratin 18 promoter; (2). Microinjection these plasmids which are listed above into the zebrafish embryos; (3). Creating two germlines for mimicking autosomal dominantly basal cell carcinoma syndrome and sporadic basal cell carcinoma; (4). Using cryosection and immunohistochemical staining to further confirm the germlines; (5). Amplification of the cancer fish lines; The second year: (1). Amplification of the cancer fish lines; (2). Using cryosection and immunohistochemical staining to further confirm the germlines; (3). Using antibodies staining to study the endogenous oncoprotein levels; (4). Microarray analysis; (5). Discovery the molecular mechanisms of autosomal dominantly basal cell carcinoma syndrome and sporadic basal cell carcinoma. The third year: (1). Amplification of the cancer fish lines; (2). Screening for the novel ruthenium-derived compounds; (3). Phenotypic observations of the embryos after treated with ruthenium-derived compounds; (4). Using antibodies staining to study the endogenous oncoprotein levels; (5). Using cryosection and immunohistochemical staining to further investigating the germlines; (6) Screening for the other small chemical library to find novel anti-cancer leading drugs.
description: 計畫編號：NSC96-2313-B032-001-MY3

新穎啤酒酵母菌蛋白質甲基轉移脢YJR129Cp之探討

title: 新穎啤酒酵母菌蛋白質甲基轉移脢YJR129Cp之探討 abstract: 本計劃是以本實驗室先前的發現為基礎。此發現已於C012-1中描述，證實了啤酒酵母菌之YJR129Cp為一真實具有活性之新穎蛋白質甲基轉移酶。此新發現利用一維電泳分析顯示，其蛋白質受質之分子量為95 kDa, 72 kDa, 55 kDa, 43 kDa，pI值為 6~7。本計劃將進一步利用蛋白質體學方法找出YJR129Cp之蛋白質受質；即進一步利用二維電泳分離及質譜分析來鑑定出蛋白質受質種類。並將利用定點突變、基因剔除、及甲基化反應等方式來證明是否YJR129Cp於體外及體內皆具有專一的活性。最後，計劃將延伸至功能方面的探討，此部分將借助於雙雜合篩選及合成致死基因篩選等技術。 This proposal is based on the previous finding in our lab that YJR129Cp of Saccharomyces cerevisiae is a bona fide, novel protein methyltransferase, as presented in C012-1. This new discovery indicates that the protein substrate has a molecular weight of 95 kDa, 72 kDa, 55 kDa, 43 kDa, and pI 6~7 in one-dimensional electrophoretic analysis. Here, identification of the protein substrate is planned by the proteomics approach; that is, two-dimensional electrophoretic separation followed by mass spectrometry. The specificity of both in vitro and in vivo activity of YJR129Cp is also planned to be verified using site-directed mutagenesis, gene deletion, and both in vitro and in vivo methylation assay. Finally, the study will be extended to the functional aspect by two-hybrid screening and synthetic lethal screening.
description: 計畫編號：NSC97-2311-B032-001

轉錄因子NF-Y, Capsulin與Musculin參與顏面肌肉軟骨發育之分子機制探討

開發基底細胞癌之斑馬魚品系用以篩選新型釕金屬錯合物之抗腫瘤前導藥物

幾丁類物質於生產細菌幾丁質分解酵素及寡醣製備之應用

微生物所生產抗菌幾丁質脢之固定化及其於生物製劑與寡糖生產之應用(II)

title: 微生物所生產抗菌幾丁質脢之固定化及其於生物製劑與寡糖生產之應用(II) description: 計畫編號：NSC93-2313-B032-001

微生物所生產抗菌幾丁質脢之固定化及其於生物製劑與寡糖生產之應用(I)

title: 微生物所生產抗菌幾丁質脢之固定化及其於生物製劑與寡糖生產之應用(I) description: 計畫編號：NSC92-2313-B032-001